Free College Essays - The Role Model in Huckleberry Finn :: Adventures Huckleberry Huck Finn Essays

Huckleberry Finn: His Role Model        Imprint Twain's The Adventures of Huckleberry Finn is composed from the view purpose of the kid Huckleberry Finn. He tells about the undertakings he is having on the Mississippi River with a runaway slave, whose name is Jim. It gets obvious right off the bat in the book that there are several individuals who assume significant jobs throughout Huck's life. One is Jim and the other is Tom Sawyer, the individual Huck wishes he could resemble. Tom Sawyer is a pioneer to Huck from the earliest starting point of the book, when Huck is living with the Widow Douglas. She is raising Huck since his dad is a tanked and isn't in the territory. Huck is doing fine living with the Widow Douglas for a little while, however he before long feels worn out on her lifestyle. Huck doesn't care for remaining clean constantly and wearing perfect garments. He likewise doesn't value her endeavoring to edify him, so he puts on his old clothes and leaves. Tom Sawyer is the person who can persuade Huck to return to the widow and be good (p. 1). Huck needs to be a piece of Tom's group, so he consents to return. It takes a specific kind of individual to make Huck ready to return home since it is a way of life he truly doesn't care for. Tom has that sort of power over Huck's choices. Another explanation that Huck admires Tom as a good example is that Huck feels Tom is more keen than himself. Huck is astounded by how splendid Tom is. What a head for only a kid to have! On the off chance that I had Tom Sawyer's head I wouldn't exchange it off to be a duke, nor mate of a steamer, nor comedian in a carnival, nor nothing I can consider (p. 236). It isn't just that Tom is more astute. It is that Tom frequently causes Huck to feel he isn't as savvy. One model is the point at which the two young men are attempting to free Jim. Huck doesn't comprehend why they need to do things the most difficult way possible. That is when Tom says, Gracious, shucks, Huck Finn, on the off chance that I was as uninformed as you I'd keep still (p. 243). Tom additionally says, Why, hain't you at any point read any books whatsoever? (p. 242). The facts confirm that Tom has more tutoring than Huck, and this additionally assumes a job into Huck's conviction that Tom is more intelligent.

How Women are Represented in Hip Hop Videos Essays

How Women are Represented in Hip Hop Videos Essays How Women are Represented in Hip Hop Videos Paper How Women are Represented in Hip Hop Videos Paper Hip Hop Videos For Years, video young ladies have been a significant piece of hip bounce music and recordings, yet what is the expense? Are young ladies losing themselves in the fabulousness and glitz of being a video young lady? This video covers Just that: what Image ladies are giving out by being in these Hip Hop recordings. These perspectives originate from a couple of various individuals who all have various jobs in the Hip Hop world. The video additionally discusses the negative jobs, for example, strolling blind, that young ladies decide to take on as they be in Hip Hop recordings. The video opens by Introducing who will be imparting their insights all through the video. It acquaints us with Melissa Ford, who is a model, Irvin Got, who is a record official, Kenned G, who is a radio show host, and Kevin Powell, who is an essayist and extremist. The main point they spread is strolling blind. The host opens by demonstrating instances of how ladies are appeared In hip jump music recordings. She investigates cuts from both a 50 penny video and a Jay Z video. The two recordings show females in little garments, out numbering the guys in the recordings. This fortifies the hosts point that the young ladies may be taken a gander at as strolling blind. As she states, they might be taken a gander at as simply decorations; much Like Jewelry. Females In music recordings are here and there utilized Just as an article. They speak to a prize or something that can be acquired once you arrive at a specific degree of fame. As indicated by http://Neolithic. Silent. Mother/2012/10/21 the-portrayal of-ladies in-hip-bounce and-rap-recordings/, Women are frequently there as unimportant sexual articles, moving provocatively in uncovering apparel, exhibiting sexual Innuendos with their bodies developments, present in the clasps completely for the hetero male craftsmen and ewers fulfillment. With that being their essential, and basically just motivation behind being in the video, they are less out and out people, as much as they are viewed as articles to be utilized. They likewise are seen just as diversion, as indicated by Irvin. Additionally, they contend that occasionally the ladies CHOOSE to place themselves in this position. A few Women imagine that being in a hip bounce video is enabling to them. They feel the requirement for the additional consideration that joins being on screen. In this video, every one of the four present speakers gives their very own dusts room various perspectives. Having various occupations in the business, they all have various thoughts, regardless of whether just somewhat. They all have various plans to share and remarks to add to the conversation. After the host presents strolling blind, the first to speak Is Irvin Got. He talks about the diversion part of the hip bounce recordings and the ladies in them. He says, Our Job is to engage You think 1 OFF ten man won Is a police officer or ten trash collector, or ten cases educator People won have genuine lives and genuine Jobs, you think [they care] ? They Just need to be engaged. This shows he isn't taking a gander at the circumstance as if ladies are being typified or given a negative picture; He considers it to be carefully a business of amusement. The females in his brain speak to something to take a gander at, and something that may potentially light up a working keeps an eye on day. Irvin even ventures to state that he would permit his own girl to be separated of one of his music recordings. This demonstrates how understood the brain of society has become, on account of the normality of these pictures in the media. In any case, this Just adds to the acknowledgment cap Irvin doesn't see it in a negative manner, and may not see the negative picture that the video young ladies are given. The second to talk is model, Melissa Ford. The host asks Ford, Have you at any point felt slighted? Have you at any point felt externalized or awkward? How is that experience? Portage reacts by saying, as far as I can tell, I took a gander at it as a Job. This is a check. This is my venturing stone to get to another level This is additionally another way you can see this circumstance. Moving in music recordings might be the main choice a few females need to get a check. Tragically, they are as yet given a negative picture and took a gander at in an awful light. She mentions that wh ile a few females do it for the business perspective, some of the time being before the camera causes a young lady to feel great about themselves. Different occasions they basically need the consideration they get from being on the camera. This additionally opens the entryway for youthful, shaky young ladies to turn into a piece of this world. A little youngster may be sitting in front of the TV and see a more established ladies moving in a provocative manner, half stripped, on a hip bounce music video. That little youngster may not consider the negative picture that model is giving herself, UT rather may see a lady who is OK with herself and is getting the consideration of many folks and much other ladies. Melissa advises that the opposition to be seen is increasingly extraordinary at this point. Where as there used to be five or six young ladies, presently theres fifty or sixty young ladies Youve got the chance to battle for the consideration. The host at that point poses the inquiry, How have you seen a change after some time Compare music recordings from the early asss to music recordings from 2007. The inquiry is focused at Kevin Powell, and he initially reacts with, muff know, from 85 to 90, 91, you didnt see that. You saw a couple of females yet there was an equalization that was going on. This shows after some time, there have been changes in the public arena that likewise change the picture that the young ladies in the hippo recordings are depicting. While previously, they may have been Just to adjust a sexual orientation proportion, presently they young ladies dwarf the folks. The young ladies are no longer taken a gander at in a similar blameless manner; they have taken on pr ogressively sexual jobs and are found in an entirely unexpected light. This is the reason the depiction of ladies in hip jump recordings has changed, and is currently negative. Powell at that point shares how he was really stunned, and thought about what sort of video is this? He hits on the point that I raised before by saying, young ladies really accept this is the thing that ladies hood is. The parity change has left young ladies believing that a video young lady is everything they can be, and their solitary way to being acknowledged and effective. The young ladies dont see the negative part of the video young lady, just that they are getting consideration by the folks around them. In conclusion, the host inquires as to whether she accepted ladies were being typified. Kenned rapidly replay, Tolerantly 1 00. I AAA nave an issue Walt ten observation Tanat Is going on with ladies in recordings today At the point when you see an excellent lady, and I regard her for being wonderful, however my concern is shouldn't something be said about the young lady that doesnt realize that alright, Just being delightful isn't sufficient. Video young ladies are radiating the observation to little youngsters that hello, in the event that youre quite, at that point this is the thing that you will get the chance to do. An ever increasing number of little youngsters are being sucked into this world, not understanding what theyre getting themselves into. Kenned takes advantage of the shallow part, all things considered, when she says, If that young lady increases twenty pounds shes out of a Job. So my issue is the reality of how these ladies are being depicted in these recordings. Little youngsters see provocative moving and attire in these music recordings and think it is alright, in light of the fact that its everything over TV and everywhere throughout the media. They feel as though this is the standard. Taking everything into account, while young ladies are Jumping at the opportunity to be in a hip bounce video as one of the numerous video young ladies, they have to initially think what they are depicting themselves as. Any young lady that is moving in the direction of being a piece of the music video world is in danger of having a terrible picture starting there on. They should take a gander at whether they are assuming a job in the belittlement of ladies, or in the event that they are basically playing out work.

Want and Ambition

1. What spring in to your psyche when you hear the word â€Å"ambition†? At whatever point I hear the word desire as though there’s somebody next to me and advising to take activities! I have such huge numbers of desire in life that I have to satisfy. These aspirations will assist me with striving harder and arrive at the objective to be a fruitful one. 2. Is it accurate to say that you are driven? Indeed, I am eager. Unquestionably I am, we as a whole need to acknowledge the realities that individuals intended to be yearning to take more diligently or endeavor to get what we objective throughout everyday life. Related article: My Aim Is To Become A CollectorPeople who are â€Å"ambitious† are seen some of the time as either egotistical or ridiculous. Yet at the same time regardless of what we look like on it, there are no such terrible things in having desire. We should simply let it out to ourselves and give ourselves authorization to seek after it. 4. Who is the most yearning individual you know? I am eager individual; I have numerous aspirations in life that I have to satisfy. I knew inside me that I have numerous things I need to have or accomplish. In family, vocation and even in popularity there are a great deal of these desire in my life. 5.What were your desire when you were a youngster? At the point when I was little, I was dreaming to turn into a columnist. At whatever point I hear somebody writing about TV I will look before the mirror and will hold anything and imagine that it’s a receiver and begin rehashing the message that the correspondent expressed on the new s. Later on I concluded that I might want to manufacture a major shop where children would have the option to discover all that they required so as their fantasies would work out as expected. My folks were continually grinning at me, enchanted by my high aspirations in life.While developing, I began to shape moreâ definite picture of my futureâ and was practically certain that I need to be an ambassador. 7. What desire do you have that you think you'll understand and won't understand? 8. For what reason do you have desire? I have my desire on the grounds that, having aspiration in harsh conditions such as things are not working out in a good way, will lead me to get myself out of them and discover joy and achievement. In the event that we have no aspiration in our life, we have no drive for anything. Nothing will appear to be significant. 9.What’s the diverse among desire and a fantasy? To dream is without exertion as though your solitary dreaming for an outlandish thing to occur while aspiration you need to make move by venture to arrive at every one of your desire and face challenges to satisfy these. 10. What aspiration have you held the longest? The desire I held the longest, in actuality, is to graduate in an ambassador with a degree holder. Wherein I can get a decent line of work to support my folks and furnish them own home and live with a cheerful and mollified life. 11.What occurs after you have satisfied every one of your desire? The best is the point at which the wellspring of aspiration turns into my longing to arrive at my large objective or to satisfy my life in reason. The main thing that will occur subsequent to satisfying my aspiration is happiness. 12. What do you think it feels like to satisfy a lifetime aspiration? I think it feels like I am the most joyful individual and will having a serene brain. Obviously everybody is asking and endeavoring to arrive at a lifetime aspiration. Furthermore, if that happens to me and in Godâ€℠¢s will, I’ll say thanks to him.

Oral fluid testing device

Abstract CTLS as part of the collaborative xxxxx Trading Ltd for validating One Step Multi-Drug Oral Fluid Drug Testing Device. Also the DOA test device registered with medicines and healthcare products. Validation study of one step multi device was performed in CTLS by two ways. Abbreviations g/L Microgram per litre QA Quality Assurance POCT Point of Care Test MHRA Medicines Healthcare products Regulatory Agency UKNEQAS United Kingdom National External Quality CAP Clinical Pathology Accreditation CTLS Clinical Trials Laboratory AMP Amphetamine BENZO Benzodiazepines COC Cocaine THC -Marijuana MAMP Methamphetamine MOR -Morphine OF Oral Fluid GC Gas Chromatography GC/MS Gas Chromatography Mass Spectrometry LC Liquid Chromatography CE European Conformity DDS Digital data storage 0C Degree centigrade SOP Standard Operating Procedure IVDMD In-Vitro Diagnostic Medical Device Validation method 1 Validation method (1) was conducted from June 2009 through to July 2009. Seven samples were collected from UKNEQAS, which were offered pilot surveys for drugs of abuse in oral fluid and tested using a one step multi device. Three samples were detected by the one step multi device. The one step multi drugs testing device test result was compared to UKNEQAS analyst report. Validation method 2 Validation method (2) started July 2009 and was completed in August 2009, eighteen urine and saliva sample were collected from volunteers and tested for drugs of abuse and tested using one step multi and Dipro Drug lab Multi panel Drug Test devices. During the test, three samples were providing positive results. The test results were compared with each method, and test result would be kept confidential so that clients information would not be disclosed for any other purpose. Chapter: 1 1 Introduction The goal of this project at CTLS was to validate the Drug Abuse Point of Care Testing Kit which will have immense value to the public as well as to the medical research. The validation of testing the kit will certainly in turn become a reliable resource for definitive screening tool in the future. Our aim was to establish clinical cross validation trials necessary to prove drugs of abuse point of care testing kit for human saliva. It could be used as an advanced, accurate and handy clinical laboratory technology to evaluate individuals drug abuse level. Our validation was a team effort to achieve the goal to use human saliva as a non-invasive-bio fluid. The saliva determines the status of a human body. In other words, it is a mirror of the body. The human saliva is easy to collect and can be obtained at low cost in sufficient quantities for analysis. Saliva based tests are more accurate, have a quicker turnaround time and less expensive, particularly as a home drug testing kit. One Step Multi-Drug Oral Fluid Test kit is ideal for qualitative determined level of Amphetamine, Benzodiazepines, Cocaine, Marijuana, Methamphetamine and Morphine in human saliva. This kit provides only preliminary screening of the results within three (3) minutes, and provides the amount of all the drugs present in the human body. If positive result occurs, confirmation must be obtained by more specific chemical method utilizing GC/MS or LC/MS. The drug of abuse of POCT kit will detect, whether any drug is present in oral fluid below the cut-off limit. 1.1 One Step Multi-Drug Oral Fluid Test kit Cut-off Concentrations. Symbol Test Calibrator Point of Care kit Cut-off level(ng/ml) AMP Amphetamine D-Amphetamine 50 BENZO Benzodiazepines Benzodiazepines 30 COC Cocaine Cocaine (parent) 20 THC Marijuana 9-THC(parent) 50 MAMP Methamphetamine D-Methamphetamine 50 MOR Morphine Morphine 40 1.2 The following are the advantages of saliva-based drug of abuse point of care testing kit: Reliable diagnostics and quick turnaround time. Easier than other test methods, which mean no additional equipment and operator training is required. Safer to use, and cheaper than current oral fluid used methods. For example other Oral Fluid methods: GC/MS, ELISA, Point of Care lab tests. Testing kit can be kept at room temperature. Testing provides an accurate and reliable assessment. This test detects most of the common drug abuses. 1.3 Reasons for using the Saliva based Drug Testing Kit: For individual who can check for themselves if there are any drugs presents in their circulation system. Testing can help the employers to determine if any of their current or potential employees abuse any of the banned (illegal) substances. Non-invasive.[19] Sample cannot be adulterated. CE mark. (European Conformity) 1.4 POCT method of DOA test compared with other DOA Test methods Method POCT Oral Fluid ELISA POCT urine GC/MS Sample Oral Fluid Oral Fluid/Urine Urine Oral Fluid, Urine, Blood, Hair Format Lateral Flow ELISA Lateral Flow GC /MS Test time 5min 48-72 hours 5min 3-7days Additional Equipment Required None Incubator ,ELISA reader None Spectrometer Storage RT 2-8 0C RT NA Shelf Life 18 months NA 24 months NA Advantage Accurate reliable Easy to use at any time and any where Non-invasive, No additional equipment required, cheap Accurate reliable High specificity Less expensive Instrument-based testing ,Accurate Disadvantage More expensive than rapid urine testing Expensive, Complex method Sample can be easily adulterated Very expensive 1.5 Oral Fluid Oral fluid is referred to as saliva. The saliva is excreted primarily by 3 glands namely, parotid, sub maxillary and sublingual. It has low protein content and can vary in flow rate from zero to several milliliters depending upon influence from various factors including emotional state and hunger. Spitting or expectoration provide neat saliva but is relatively viscous, and can be difficult to work within the clinical laboratory. It may also be contaminated with food and other particles from the mouth and will therefore require centrifugation. Some of the collection methods used by various companies are those indicated in Table 🙠 1.8.5) and they use some forms of proprietary diluent to mix with collected saliva. The absorbent pad or foam is used to collect saliva comparatively there is no one type of collection device that is better than other when it comes to ease of use. However, there are continuous developments of new products. [6] Plasma electrolytes such as bicarbonate, chloride, potassium and sodium and other plasma elements such as enzyme, immunoglobulin and DNA are present in saliva produced from three major and several minor salivary glands. Plasma electrolytes such as bicarbonate, chloride, potassium and sodium and other plasma elements such as enzyme, immunoglobulin and DNA are present in saliva. Most of drugs can be detected in saliva; including amphetamine, barbiturates, benzodiazepine cannabinoids cocaine methamphetamine ethanol and heroin. [1] However many factors may affect drug transfer into saliva, such as degree of protein binding, pKa, physical size and lipophilicity of drug. 1.6 Advantages of saliva as drug testing matrix Nowadays saliva matrix is more popular for screening of drugs of abuse .The advantages of using saliva matrix are provided below. Saliva collection is easier than other biological fluid. Also direct collection is not embarrassing and significantly reduces an influence of interference enter into sample collection process. Blood collection is a highly invasive method. Testing is more reliable and adaptable for roadside testing. Samples are analysed directly without any further pre-treatment and test results are provided immediately, for example the new brand of Cozard DDS system can provide results within ninety seconds for two major classes of drugs and less than six minutes for six other major classes of drugs. This can help to initiate law enforcement process with sufficient data. An interpretation of drugs in saliva can be used to determine pharmacokinetic limitation. Therefore a low analyte concentration can be easily detected. 1.7 Disadvantages of saliva as drug testing matrix Concentration of drug can be changed by pH during the sample collection. Therefore Saliva and Plasma ratio may be changed. [8] 1.8 Saliva collection methods Production of saliva is influenced by a variety of methods such as stimulating and non-stimulating collection of saliva. [9, 4] 1.8.1 Stimulating method Saliva can be swabbed from mouth using absorbent materials such as sour coated cotton balls. The absorbent material of cotton ball becomes soaked with saliva, which can be removed from the mouth and the saliva is separated by centrifugation or applying pressure to the cotton ball. [3, 4].However, saliva collection method by stimulation is connected with several potential problems, for example the absorbent material can react or absorb some drugs. Therefore actual amount of drug concentration can be varied in saliva. Also some salivary stimulate material (sour flavor) may change the saliva pH level. For example, the level of codeine present in saliva collected after stimulation, which can be decreased by an average of 3.6 times of non-stimulated method of collection. [5].Also non-stimulated saliva contained substantially more drugs than stimulated [8]. 1.8.2 Non-stimulating method Saliva can be collected from mouth into a collection device without using any salivary stimulate material .However, some physical techniques are used to produces saliva ,such as tongue, cheek and lip movements. [8] 1.8.3 Problems with Sample collecting device of saliva Most of sample collection devices contain a form of an inert absorbent pad for saliva collection [9]. However, different amount of saliva is absorbed during the sample collection process by different devices. Collection of sample is not assured by most of sampling devices. Therefore many of devices have a colour changing indicator. An absorbent pad made from different materials. This material must be chosen carefully because some materials may absorb some of the drug. This can lead to errors. Different buffers /preservatives which used in different devices may be incompatible with hyphenated MS techniques for a confirmation of drug abuse levels [4]. Some of the most common problems: ion suppression in LC-tandem MS long term contamination of columns and ion source in GC 1.8.4 Advantages of sample collecting devices The point of care saliva collector uses an optimal method for obtaining full saliva. Saliva is easily obtained by chewing on a roll-shaped saliva collector and Very Cheap. The chewable kit are coated with citric acid .This can be stimulated more saliva. 1.8.5 The list of saliva sample collection devices and characteristics Collection Device Made in Method of collection Control of, saliva volume Buffer /diluents Stimulated collection Cozartoral swab. UK Absorbent swab Yes Yes No Intercept Device. USA Salt impregnated pad No Yes Yes Drug Wipe. Germany Absorbent pad Yes No No Greiner Bio-One. Austria Saliva extraction solution Yes Yes No Saliva Twist device Drug Test. UK Sponge swab No Yes No Oralab 6. USA Absorbent pad Yes No No Oraline. USA Collection cup Yes No No Oral Stat. USA Sponge swab No Yes No Oratect. USA Absorbent pad Yes No No VerOFy.USA Absorbent pad Yes No No Saliva Screen. Germany Absorbent pad Yes Yes Yes, acidic available mechanical Smart clip multidrug. Germany Absorbent pad No Yes Yes, mechanical Quantisal. USA Absorbent pad Yes Yes No Salivette. Germany Cotton wool/polyester swab No No Yes, acidic available mechanical Source: young scientist journal June 2009 and the kit manufactures web sites 1.9 Literature Reviews (1) Biermann et al., 2004 did research on On-site testing of illicit drugs using Toxiquick. The experiment was carried out for 18 months. During the periods 751 individuals were tested at road site with immunochemical test device Toxiquick for drug abuse such as amphetamine, MDMA, MDE, cannabis, cocaine, heroin and morphine. 302 individuals oral and blood samples were taken during the periods. This was tested with immunochemical and non immunochemical method (GC-MS) and the test results were compared. In general 75% Toxiquick results were agreed with GC-MS results. However, cannabinoids test were shown to have negative values. This can be caused by metabolise. (2)Vanna De Rose et.at(2004) wrote a review report on drug testing in the work place, and the summary of their review report is given below directly quoted from their work. Drug testing in the work place: There are various methods of approaches to identify drugs of abuse for staff . For example urine, blood, oral fluid, hair and sweat can be used to test. Four fundamental reasons concern the author, for drug testing at work place; the cost of drug testing at work place, how reliable are the results? Cut off levels and strength of drug testing device. Safety: drugs of abuse can increase safety risk to own self and co-workers at work place, for example, where employees are working at higher sensitivity working area such as air traffic controllers, Pilots and Ambulance drivers etc. Organizational efficiency: drugs of abuse can increase low productivity, high staff turnover and absenteeism. Therefore drugs testing can increase the productivity and reduce the cost of new employees and training them. Reputational risk: When an employee takes an illegal drug, the reputation of the organization can be damaged. Employee welfare: Drug test can improve employee health and would help to identifying staff that need to be sued for drug intake. The cost of drug testing at work place can be influenced by three major factors. Higher financial costs are involved for drug test. Drug testing can be damaged relationship between staff. Employment of staff and human resource service are a significantly higher cost. Testing device can detect drugs other than illicit drugs. For example over-the-counter painkillers can be detected and have produced a false positive result because of the opiate. This problem can cause discrimination against a person who is accused of false impairment. The problem can be resolved by changing of identification cut off limits for specific level of illicit drugs. Most of drug testing device can indicate only drug metabolite activity in body but not the level of intoxication. (3)E.J. Cone. et.al (2007) in their paper on Interpretation of oral fluid test for drugs of abuse The article reviewed several aspects of oral fluid testing, detection times, application of oral fluid testing and interpretation of test result .They also reported the influence of chemical, physiological and pharmacological factors that are involved in oral fluid testing result. (4)Prof Olaf H Drummer. Clinical Biochemist Reviews (2006, August 27(3): 147-159) In The Clinical Biochemist Journal the application of method of testing for drugs in saliva such as LC-MS, HPLC with MS are compared with road site drug testing kit. The Advantages of testing kit were described, in the Journal, to be a level of accuracy and efficiency. (5)Kato et al .J Anal Toxicology 1993 Oct: 17(6):338-41 Cocaine and metabolite excretion in saliva under stimulated and non-stimulated conditions .The collections of saliva sample were carried out by stimulated or non-stimulated condition from six healthy volunteers who were given cocaine during the test session. The presence of cocaine level and metabolites were analysed by GC-MS .They reported that the non-stimulated saliva contained more cocaine than stimulated. This can be caused by levels of pH present in saliva. (6) Dr.Graham Facile. Evaluation of the Drager Drug Test 5000Test System. (2008): Drager Drug Test 5000 test system has been used for detecting drugs in human-saliva /oral fluid. During the period of testing, 503 individual tests were done .The test results obtained were within minutes utilizing Drager Drug Test Test System .Also this device can detect the trace level of drugs present in saliva, for example, Cocaine (20ng/ml), Opiates (20ng/ml), Benzodiazepine (15ng/ml), Delta -9-THC (25 ng/ml) and Amphetamine (50 ng/ml).The results were compared with drugs of abuse point of care kit cut- off values. The Drager Drug Test 5000 Test System was found as more powerful immunochemical based diagnostic medical device. (7) N.Fucci et.al SPME-GC analysis of THC in saliva sample collected with EPITOPE device. (2001).The article mentioned several factors about oral fluid analysed with different techniques, method of collection device and oral fluid reflection for drug concentration. They reported that the drug concentration in saliva is directly reflected to plasma concentration in body. They detected a higher level of cannabinoid in saliva. So, this could be due to the fact that cannabinoid smoke is directly saturated in the oral cavity. They used an EPITOPE device, which has an absorbent pad or foam used to collect saliva. (8)O.R.Idowu and B.Caddy A Review of the use of Saliva in the forensic detection of drugs and other chemicals (2008).In their review they reported the saliva sample to be useful for drug detection in the forensic analysis. Concentration of drug level in Plasma can be reflected in saliva. (9)Schramm.W et.al Drugs of abuse in saliva Journal of Analytical Toxicology (1992) This article reviewed various aspects of drugs of abuse in saliva. Analytical methods were used for the detection of drugs, including gas chromatography with mass spectrometry, thin layer chromatography and immunoassay. The study included that the level of concentration of cocaine and phencyclidine is correlated with saliva and plasma. They also found that the drug concentration of tetrahydrocannabinol (marijunana) can be reflected in saliva and blood. (10)Dennis .J.Crouch et.alAn Evaluation of innovation Sweat Based Drug Testing Techniques for use in Criminal Justice Drug Testing April 2002 The article discussed the acceptable drug/metabolites confirmations techniques such as GCMS / LCMS and LC/MS/MS. However, the researcher pointed out that the cannabinoid (THC) drug analysed with LC/MS/MS, can cause some interference, such as, electron spray ionization due to lack of a functional group ionized made electron spray. (11) Creative Research Systems. https://www.surveysystem.com/sscalc.htm. National Statistical Service. https:// www.abs.gov.au These articles provide the relationship between the sample numbers, confidence level and the confidence interval. Chapter: 2 2 Validation Method This study of saliva matrix based on drugs of abuse point care testing kit [DAPOCTK] validation was performed in CTLS using two methods. 2.1 Validation Method: 1 A validation of Drug Abuse Testing Point of Care Kit [DAPOCTK] was performed by participating in accordance with United Kingdom National External Quality Assessment Scheme [UKNEQAS] for oral fluid Drugs of Abuse. 2.1.1 Role of United Kingdom National External Quality Assessment Scheme [UKNEQAS] The UKNEQAS can offers a number of proficiency testing schemes for monitoring drug assays in serum, pilot surveys for drugs of abuse in saliva toxicological investigations and drugs of abuse testing in urine. Also a quality assurance service with strong educational knowledge is provided. 2.1.2 How does the Pilot Surveys Scheme work? Known coded samples will be distributed quarterly and drug analysis will be performed for 4 weeks. The analytical performance will be informed to participated laboratories. 2.2 Validation Method: 2 The validation purpose of this study, urine and saliva specimens were required from volunteers and tested for drugs of abuse with one step multi and Dipro Drug lab Multi panel Drug Test devices. 2.2.1 Outcome of Comparison of two POCT aid Validation: Both CE marked POC testing kit are ideal for qualitative determined level of drugs of abuse in human bodies using human biological specimens. Saliva matrix based on POC testing kit results correlate with urine matrix POCT kit result. The Saliva matrix based on POC testing kit will be validated by urine matrix POCT kit result. 2.2.2 The voluntary participation with CTLS for validation of POCT kit purposes: The identities of participants will not be disclosed or divulged to the scientist / other staff members of CTLS who receive sample of oral-fluid /saliva. The purpose of Drug of Abuse Information and Consent Form [Appendix II] is to provide information pertaining to voluntary participants of drugs-abuse and CTLS for research purposes as to the nature of the role and the legal and ethical standards to be met. 2.2.3 Scope of the Legalities: To protect the confidentiality of the identities of the voluntary participants any information about the participants will not be revealed. Steps will be taken to protect the confidentiality of voluntary participants data including code numbers, database, and storage of research data in CTLS. Chapter: 4 4 Registered a point of care testing kit with Medicines and Healthcare products Regulatory Agency. 4.1 Medicines and Healthcare products Regulatory Agency. The Medicines and Healthcare products Regulatory Agency (MHRA) is United Kingdom government agency of the Department of Health. 4.2 Role of MHRA MHRA is responsible for making sure that medicine and medical devices work with safety regulation in UK 2002. Medicines and medical devices information are provided so that they are publicly available. MHRA is monitoring medicines and medical devices in market and encourage the public to inform them of any problem with a medicines and medical device. If there are any problems with medicines and medical devices, then they are removed from market, so that they can be investigated and the necessary action needed can be taken against manufacturer. 4.3 In Vitro Diagnostic Medical Devices Register with the MHRA CE marking declaration certification [Appendix III] Registration Form RG3 Registration fees The required information for registration process and other pertinent information can be found in the MHRA Web site. https://www.mhra.gov.uk/Howweregulate/Devices/Registrationofmedicaldevices/CON009810 Chapter: 5 5 Method Validation Plan: Title: Validation of Drugs of Abuse Point of Care Testing kit Objective: To validate Drug Abuse Testing Point of Care Kit [DAPOCTK] by participation of the United Kingdom National External Quality Assessment Scheme for oral fluid drugs of abuse. The POCT kit will be validated using UKNEQAS oral fluid samples in Clinical Trials Laboratory Services Ltd. The POCT kit will be validated by comparison with Volunteer urine samples screening DOA test in Clinical Trials Laboratory Services Ltd. 5.1 Validation of Analytical Method 5.1.1 Reference Compound, Apparatus and POCT devices 5.1.2 Reference Compound of Oral Fluid UKNEQAS Reference Item will be analysed for validation. Identity: Oral Fluid Provide by: United Kingdom National External Quality Assessment Schemes Batch No: OF/1008/October 08, OF/0609/June 09, Oral Fluid: (OF) 5.1.3 Apparatus Micro Pasteur pipette (10l) 5.1.4 Different matrices of POCT devices 5.1.4.1 Saliva matrix based on a point of care test kit. Trade name of kit: XXXX Oral Fluid Test kit. Manufacture: XXXXX.Ltd in China Batch No: W650690510. Expiry date: 05/2011. Drug tests for: COC/Amp/mAmp/THC/MOP/BZO. CE marked by Qarad European Regulatory Service in Belgium. XXX Oral Fluid Test kit contained: Saliva Test Panel, Saliva Collection tube, Saliva Sponge Collector. 5.1.4.2 Urine matrix based on a point of care test kit. Trade name of kit: XXXXXX Multi panel Drug Test Manufacture: XXXXX in Austria. Batch No: DOA8110062. Expiry date: 01/2010. Drug tests for: COC/Amp/mAmp/THC/MTD/MOP/PCP/BAR/BZO/TCA XXXX Multi panel Drug Test kit contained: Urine Test Panel 5.2 Number of sample determination: For determinations of sample size the following factors should be considered. How accurate the result needs to be? The level of confidence limit of the test results. Available budgets for sampling and analysis. The number of samples needed for validation can be determined by two calculation methods. 5.2.1 Calculation method: 1 S = (z/e) 2 S -The sample size, Z -The degree of confidence (95%, 1.96) 5.2.2 Calculation method: 2 S = [Z2 x (p) x (1-p)] / C2 S -The sample size, Z -The degree of confidence (95%, 1.96), C Accepted error value as proportion, P An estimate of the proportion of numbers of samples falling into the group of representing the population. A larger number of samples truly reflect the population size. However, the experiment will be conducted with a minimum number of samples. Therefore the confidence interval percentage will be chosen as +/-19.6 and number of samples found as 25 at confidence level 95% [2, 10].In this validation of POCT kit experiment, the calculation method (1) will be used for determination of number of samples. 5.3 Experimental Pathways: This validation of this experiment can be carried out by two different experimental pathways. However, the total number of samples will be maintained. (n= 25) 5.3.1 Experiment Pathway: 1 Saliva based on drugs of abuse POCT kit validation process can be carried out by UKNEQAS oral fluid samples .Oral Fluid will be directly introduced in to POC testing panel, where a sample introduction place [Appendix 1 (4)]. This validation process can be taken over a period of one year. 5.3.2 Experiment Pathway: 2 Saliva based on drugs of abuse POCT kit validation process can be carried out by urine based on drugs of abuse POCT kit. This validation process will be completed in a short time frame. The short period of time will depend on availability of sample. A portion of the saliva and urine sample will be introduced into appropriate matrix of POC testing panel, where a sample introduction place. [Appendix 1 (4)] 5.4 Description of the Validation Procedure: 5.4.1 Sample collection methods: Saliva or oral fluid and urine samples will be collected from healthy volunteers or from patients taking known medication. 5.4.1.1 Saliva sample: Saliva directly collected from the mouth using saliva sponge collector. Then the saliva saturated sponge will be squashed in to saliva collector device. [Appendix: I (2, 3)] 5.4.1.2 Urine sample: Urine sample directly collected from volunteers. 5.4.1.3 UKNEQAS sample: UKNEQAS Oral fluid samples will be collected from volunteers or patients taking known medication. 5.4.2 Sample pre-treatment: UKNEQAS Oral fluid samples will be diluted with oral fluid free of all abused drug groups. The UKNEQAS oral fluid samples concentration will be adjusted for investigation of laboratory performance levels. The UKNEQAS oral fluid samples will be preserved by heating at 60 oC for 90 minutes and addition of bronidox 0.05%, gentamicin 0.1g/L and penicillin 0.1 g/L.[UKNEQAS Oral Fluid pilot surveys for drugs of abuse in saliva guide line 2009] 5.4.3 Handling procedure for POCT device and sample: All POCT devices will be operated in accordance with manufactures instruction. After use POCT device will be disposed in accordance with applicable law. All POCT devices will not used beyond their expiration dates. All biological specimen samples should be considered as potentially hazardous. All used POCT device and biological specimen can transfer infections. 5.4.4 Composition of Validation Run: Each biological specimen will be performed twice for validation run. A test result is indicated by colour or faint band in the Test Valid Window of POCT kit. 5.4.5 Sensitivity: The drug of abuse of POCT kit will detect if any drug is present in oral fluid above the cut-off limit. 5.4.6 One Step Multi-Drug Oral Fluid Test kit Cutoff Concentrations Symbol Name of Drug Calibrator POCT kit Cut-off level(ng/ml) AMP Amphetamine D-Amphetamine 50 BENZO Benzodiazepines Benzodiazepines 30 COC Cocaine Cocaine (parent) 20 THC Marijuana 9-THC(parent) 50 MAMP Methamphetamine D-Methamphetamine 50 MOR Morphine Morphine 40 5.4.6 Specificity: A stimulating saliva collection method is connected with several potential problems, for example, the absorbent material can react or absorb some drugs. Therefore, the actual amount of drug concentration can vary in saliva. A salivary stimulate material (sour flavor) may change the saliva pH level. For example, the level of codeine present in saliva collected after stimulation can be decreased by an average of 3.6 times of non-stimulated method of collection. [4].Therefore, non-stimulated saliva contains a substantially higher amount of drugs than stimulated saliva. [8]Testing devices can detect other than illicit drugs. For example, over-the-counter painkillers can be detected and have produced a false positive result because of the opiate [15] A higher level of cannabinoid is detected in the saliva. This could be due to cannabinoid smoke being directly saturated in the oral cavity. [9] The level of concentration of cocaine and phencyclidine is correlated with saliva and plasma. Also it was found that tetrahydrocannabinol (marijuana) drug concentration can be reflected in saliva and blood. [13] 5.4.7 Limitation of Procedure: This One Step Multi-Drug Oral Fluid testing kit is designed for detection of drugs in human saliva only. A positive result will be visibly obtained on test window in the kit. Therefore only the presence of a drug is found in testing sample. However, the test result can indicate a false result due to technical or procedural error. This testing kit cannot be measured or indicate the intoxication level in the body and distinguish between drug of abuse and medicines. 5.4.8 POCT device and Sample Storage: A drug of abuse testing kit will be kept at room temperature (15-30oC) in the original foil pouch. Saliva or oral fluid will be stored at 4oC in refrigerator for up to 2 days or frozen at -20 oC until use. 5.4.9 Interpret Test Validity and Test Result: Test validity and result will be interpreted within 5 minutes after initiating the test and result confirmation will be reported within 10 minutes. 5.4.9.1 Interpret Test Validity: The test validity is indicated by formation of a colour band in the POCT device Test Valid window. However, the development of colour bands may require up to 15 minutes in the Test Valid Window. This can be caused by the high viscosity and variability of saliva sample. 5.4.9.2 Interpretation of Test Result: The test result will be interpreted as either negative or positive immediately. However, the random error of visual test result interpretation will be determined by three analysts. If three individuals visual test results will be found as significantly different, confirmation must be obtained by more specific chemical method using GC/MS or LC/MS. 5.4.9.2.1 Positive Test Result: There is a positive result when the coloured band cannot be observed in test valid window next to the specified drug name. Therefore specified drugs can be found in saliva above the cut off limit. However, one coloured band observed in the control valid window, has to be present for the results to be valid. 5.4.9.2.2 Negative Test Result: Two coloured band can be observed. One colour band can be observed in test valid window next to the specified drug name and the other colour band can be observed in control valid window. 5.4.9.2.3 Invalid Test Result: Coloured band cannot be observed in control valid window. Chapter: 6 6 Result: 6.1 Table R: 1 Results for volunteer saliva and urine samples screened with POCT. Sample Saliva Sample Screening with POCT Result Urine Sample Screening with POCT Result ID AMP BENZO COC THC mAMP MOP AMP BENZO COC THC mAMP MOP 0330661 N N N N N N N N N N N N 0330662 N N N N N P N N N N N P 0330644 N N N N N P N N N N N P 0330670 N N N N N N N N N N N N 0330675 N N N N N N N N N N N N 0330691 N N N N N N N N N N N N 0330692 N N N N N N N N N N N N 0330701 N N N N N N N N N N N N 0330702 N N N N N N N N N N N N Note: AMP: Amphetamine, BENZO: Benzodiazepine, COC: Cocaine, THC: Marijuana, mAmp: methamphetamine, MOP: Morphine, P = positive, N = negative, U=uncertain or numeric result. OF: oral fluid 6.2 Table R: 2 Results for UKNEQAS oral fluid (Oct 2008) sample screened in CTLS with One Step Multi-drug POCT and UKNEQAS Analyst Report. Sample Oral fluid sample Screening in CLTS with POCT UKNEQAS analyst result report ID AMP BENZO COC THC mAMP MOP AMP BENZO COC THC mAMP MOP OF11008 P N P N N N P N N N N N OF21008 N N N N N N N N N N N N OF3 1008 N N N N N N N N N N N N OF4 1008 N N P N N N N N N N N N Note: AMP: Amphetamine, BENZO: Benzodiazepine, COC: Cocaine, THC: Marijuana, mAmp: methamphetamine, MOP: Morphine, P = positive, N = negative, U=uncertain or numeric result. OF: oral fluid 6.3 Table R: 3Results for UKNEQAS oral fluid (June 2009) sample screened in CTLS with One Step Multi-drug POCT and UKNEQAS Analyst Report. Sample Sample Screening Result in CLTS UKNEQAS analyst result ID AMP BENZO COC THC mAMP MOP AMP BENZO COC THC mAMP MOP OF10609 N N N N N N N N N P N N OF20609 N N N N N N N P N N N N OF30609 N N N N P N P N N N N N Note: AMP: Amphetamine, BENZO: Benzodiazepine, COC: Cocaine, THC: Marijuana, mAmp: methamphetamine, MOP: Morphine, P = positive, N = negative, U=uncertain or numeric result. OF: oral fluid Chapter: 7 7 Discussion During POCT validation process, there were a number of difficulties encountered in collection samples to obtain valid data. One of the problems identified was poor sampling transportation. Therefore cannabinoid group and Delta-9-THC drugs initial concentration can be decomposed or metabolized by directly contact with plastic vials as such long hours. Second problem was that when using a One Step Multi drug POCT kit detect over-the-counter painkillers, false positive result was provided due to the opiate. Volunteers saliva and urine sample were tested with saliva matrix POCT kit and urine matrix POCT kit for drugs of abuse. The results were shown table R1. Two out of six (6) sample results had morphine in them 0330662 and 0330644.One of the two test results was confirmed by volunteers medical history. The other result was not confirmed. This is because the volunteer did not disclose any information. Also the saliva and urine POCT kit did not detect for Amphetamine, Benzodiazepine, Cocaine, Marijuana and Methamphetamine in saliva. This could have been due to the presence of lower level of drug concentration in the oral fluid and urine specimens, which was below the device designated cut-off limit. UKNEQAS sample batch (2008) of oral fluid samples were tested with One Step Multi drug POCT kit in CTLS lab for specific six drugs namely, Amphetamine, Benzodiazepines, Cocaine, Marijuana, Methamphetamine and Morphine. The results were shown table R2. Amphetamine and Cocaine drugs were correctly identified by employing an immunoassay based on One Step Multi drug POCT kit in oral fluid OF1 1008, OF4 1008 respectively. In the following UKNEQAS (Nov 2008) report, eighteen out of the twenty-two laboratories tested had positive results of amphetamine in oral fluid OF11008 was reported. Ten laboratories correctly identified the amphetamine using hyphenated mass spectrometry techniques. Two laboratories results showed the detection of amphetamine, using ELISA method and the results were confirmed by GCMS techniques. Additionally, a UKNEQAS analyst was informed, 52.2g/L of amphetamine was spiked in oral fluid OF1 1008.The One Step Multi drug POCT kit cut-off value for amphetamine was reported as 50 g/L by manufacturer. Also Cocaine was detected in oral fluid OF11008 and OF4 1008 respectively by one step multi drug POCT in CTLS. In addition, eight out of twenty-two laboratories results were reported to contain cocaine in oral fluid OF4 100 8. However, the UKNEQAS analyst report (Nov 2008) mentioned no correct report has been entered for the cocaine metabolite group represented by the presence of benzoylecgonine at a concentration above that for the single analyt (8g/L) but not that for the group (20g/L). UKNEQAS sample batch (2009) oral fluid samples were tested with One Step Multi drug POCT kit in CTLS lab for six particular drugs namely, Amphetamine, Benzodiazepines, Cocaine, Marijuana, Methamphetamine and Morphine. The results were shown table R2. The One Step Multi-Drug test device gave more false negative results than valid results for cannabinoid group, benzodiazepine group and amphetamine group, from UKNEQAS oral sample batch June 2009. There were possible reasons: 7.1 Cannabinoid group: Initial, the oral sample was spiked with 19.8 ng/ml of delta-9 tetrahydrocannabinol. The sample arrived two days later by post. Therefore cannabinoid group and Delta-9-THC drugs initial concentration can be decomposed or metabolized by direct contact with plastic vials during such a long period of time. Also One Step Multi-Drug test device cut-off limit for delta-9 tetrahydrocannabinol was reported as 20ng/ml. 7.2 Benzodiazepine group: Initial, the oral sample was spiked with 11.5ng/ml of Benzodiazepine group. However, One Step Multi-Drug test device cut-off limit for Benzodiazepine group was reported as 30ng/ml. 7.3 Amphetamine group: Initial, the oral sample was spiked with 52.3ng/ml of MDMA (3, 4-methyl enedioxymethylafetamineand) 17.6 ng /ml of MDA. (Methyl ene dioxymethylafetamine) However, One Step Multi-Drug test device was made for amphetamine immunoassay only. Chapter: 8 8 Conclusion The One Step Multi-Drug test device was sensitive enough to detect the THC and term of amphetamine group, in UKNEQAS external quality assessment scheme for drugs in oral fluid (June 2009) as reported in results. The outcomes of the Validation of POCT device raise several interesting analytical problem that could resolve in further code known sample analysis. Appendix II Consent Form Drugs of Abuse Information and Consent Form Information and Consent Form for volunteers to donate Saliva, and Urine samples to Clinical Trials Laboratory Services for research purposes.Fresh human Saliva and Urine are required for research purposes by Clinical Trials Laboratory Services (CTLS) clients. These clients use the Saliva and Urine to help with basic research into various drugs of abuse. Please note that genetic research (DNA genotyping studies) will NOT be performed using these bio samples. Any remaining unused saliva and urine product will be discarded once a specific piece of work has been completed. However, in a few instances processed samples may be stored for later subsequent research. You are being asked to give consent for your saliva and urine to be used by scientists for research purposes. To protect your privacy under the Data Protection Act your sample will be labeled (or coded) only with a subject number, not your name or any other personal identifier. Some information (e.g. donor gender and age) may also be supplied with your sample in a similarly coded fashion to the scientists. Only CTLSs authorized Clinical Study Unit staff will have access to the link between your subject number and your name. In this way, your identity will not be divulged to the scientists who receive your saliva and urine sample. Your participation is voluntary; you are free to withdraw consent and leave the Volunteer Panel at any time without explanation. After each donation you will be reimbursed for your inconvenience and time. The results of any research might be valuable for commercial and/or intellectual property purposes (patenting for example). If you decide to become a volunteer, you are giving your sample to CTLSs clients who will retain sole ownership of any such research results and of any use or development of the research records (including your sample) consistent with this consent. You will not receive any financial benefit that might come from the research results. Consent My signature below indicates that: I have read and understand this form and all the information given to me. I have been given the opportunity to discuss the donation and ask questions. I am satisfied with the answers. I wish to be part of the CTLS Saliva and Urine Donation Volunteer Panel. I understand that my Saliva and Urine will be used for non-genetic research purposes. I understand that I will not be able to receive the results of any research. I will have no ownership of the research results or the research records. I agree that CTLSs clients may apply for and use patents relating to the research results, records and developments. I do give consent to take _______________ ml of saliva. I do give consent to take _______________ ml of urine. Volunteers name (Please print) Signature of Volunteer Date ________________________ _____________________ _________ Volunteer is fit for blood, urine and saliva donation. Screening Staff: ___________________Signature: ________________ Witness Name: ____________________Witness Signature: _____________________ Contact no: _________________E-mail ID: ___________________________ A.IV.3Saliva and URINE SAMPLE test result CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 07/07/2009 Lab Ref No: 0330661 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR P P Date: 07/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine .P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 08/07/2009 Lab Ref No: 0330662 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 08/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine .P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 08/07/2009 Lab Ref No: 0330664 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR P P Date: 08/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine. P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 13/07/2009 Lab Ref No: 0330670 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 13/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 21/07/2009 Lab Ref No: 0330675 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 21/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 28/07/2009 Lab Ref No: 0330691 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 28/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 29/07/2009 Lab Ref No: 0330692 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 29/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 05/08/2009 Lab Ref No: 0330701 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 21/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 05/08/2009 Lab Ref No: 0330702 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 28/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. References: Biermann. T,Schwarze B,Zedler.B and Betz.POn-site testing of illicit drugs Journal of Forensic Science International 143 (2004) 21-25. Creative Research Systems.411B Street Suite2 Petaluma CA 94952 Tel: (707)765-1001 https://www.surveysystem.com/sscalc.htm online. 30th June 2009 Dennis .J. Crouch, Dr. Royer F Cook, Dr James V.Trudea,David C.Dove An Evaluation of Innovation Sweat Based Drug Testing Techniques for use in Criminal Justice Drug Testing U.S. Department of Commerce, Office of Law Enforcement Standard.2002,206825.https://www.eeel.nist.gov. Accessed 30th June 2009 Dennis J.Crouch et.al Oral fluid collection: The neglected variable in oral fluid testing. Journal of Forensic Science International 150 (2005) 165-173. Dr.Graham Facile Cope, Honorary Senior Research Fellow. Evaluation of the Drager Drug Test 5000Test System. University of Birmingham 2008 November. Edward J. Cone and Marilyn A Huestis Interpretation of Oral Fluid Tests for Drugs of Abuse. Ann NY Acad Sci.2007 March 1098, 51-103. Jayne E. Thatcher. ROSITA II Project: Evaluation of On-Site Saliva Drug Testing Devices in Washington State.2007 Kato K,Hillsgrove M,Weinhold L,Gorelick DA,Darwin WD,Cone E J Cocaine and metabolite excretion in Saliva under Stimulated and non-Stimulated conditionsJ Anal Toxicology 1993 Oct: 17(6):338-41 N.Fucci,N.De Giovanni,M.Chiarotti,S.Scarlata.SPME-GC analysis of THC in saliva sample collected with EPITOPE device. Journal of Forensic Science International 2001 Volume 119, Issue 3,318-321. National Statistical Service, Australian Bureau of Statistical, PO Box 10 Belconnen ACT 2616, Austrialia. https:// www.abs.gov.au accessed 03rd July2009 O.R.Idowu and B.Caddy A Review of the use of Saliva in the forensic detection of drugs and other chemicals (1982) Journal of Forensic Science Society Volume 22, Issue 2,123-135. https:// www.icadts2007.org/print/80rosita_salivascreen.pdf Accessed 03rd July 2009 Prof Olaf H Drummer. Drug Testing in Oral Fluid Clinical Biochemist Reviews (2006) August 27(3): 147-159. Schramm.W.Smith RH, Craig PADrugs of abuse in saliva Journal of Analytical Toxicology (1992)16:1-9.39 UKNEQAS Oral Fluid pilot surveys for drugs of abuse in saliva guide line 2009]. http:/ www.heathcontrol.com Accessed 03rd August 2009. Vanna De Rosas,Marcus Roberts,Ruth Evans,Yolande BurginDrug testing in the work place the report of the independent inquiry into Drug Testing at work. the Joseph Rowntree Foundation. 2004 online pdf ISBN 185935212X, www.jrf.org.uk. Accessed 03rd August 2009 Vincent Cirimele, Marion Villain, and Patrick Mura Marc.Bernard Pascal Kintz Oral fluid testing for cannabis: On-site Oraline IV s.a.t device versus GC/MS.Forensic Science International 16, 2006, 180-184. Walsh J.M, Flegel R., Crouch D.J, Cangianeli L, Baudys J. An Evaluation of Rapid Point-of-collection Oral Fluid Drug-Testing Devices Journal of analytical Toxicology, volume 27,No 7, October 2003,pp.429-439(11) Jonnne Birchall. Sampling and samples Market Research World. Online article: accessed on 15th July 2009. https://www.marketresearchworld.net. Dr.Rob C.A.A van Schie,Dr Mark E.Wilson Saliva: Your Spitting Image National Institutes of Health in Bethesda, Maryland. October 1997. Online article: accessed on 15th July 2009.https://www.nih.gov/news/pr/oct97/nidr-22.htm

Essay about How Divorce is Affecting the American Culture

The effects of divorce on the American culture are immense. Social scientists have been studying these effects for many years now. The studies are continuing to confirm that the climbing rate of divorce in the American culture is hurting the society and also frequently devastating the lives of many American children. There are many areas in which divorce has a negative effect in the life of a child or an adult. Many of these effects also directly correlate to the effect on a society. However, there is hope. Although divorce is being more widely recognized as being harmful to both our culture and to the individuals involved, there are many ways that we as a culture can seek to reduce this harm and attain our goal of being a culture†¦show more content†¦This also tends to create problems in the children relating to conflict handling abilities and also poor self esteem. Children in a home with two worshiping parents have a better chance of overcoming these obstacles. 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Adelphia Communications Scandal - 1563 Words

Adelphia Communications scandal Matthew Tassin Trident University Ethics 501 Introduction Adelphia Communications Company was a television cable company whose headquarters centered in Coudersport, Pennsylvania. It ranked as the fifth most prestigious cable companies in United States. John Rigas is the founder of the company. The company was highly respected until an infamous scandal ensued following claims of bankrupt in 2002, at which time its headquarters relocated to greenwood Village, Colorado. According to Jefferson (2007), after the ensuing controversial legal proceedings ended, the company’s assets were liquefied. Time Warner Cable acquired most of the company’s assets in 2006. LFC auctioned the rest of the assets. Pioneer†¦show more content†¦The first ethical issue A company’s external editor is mandated with assessing a public company’s ability to accurately institute internal financial reporting structure and systems as well as evaluating the company to ensure that that there are no loopholes that mischievous individuals can use to fraud the company (Lowenstein, 2004). Shareholders and the public place their trust on the external auditor in revealing mischievous activities that take place in a company. In Adelphia Communication Corporation context, the shareholders and the public trusted Deloitte. They believed that Deloitte could and would evaluate Adelphia’s operations, identify and report suspicious activities perpetrated by the company. According to investigations, a number of warning signs could have notified Deloitte of the fraudulent activity at Adelphia. Therefore, Deloitte was either incompetent in performing its duty or it out-rightly falsified its report. Considering its reputation and ensuing investigation, th e latter is true. Based on AICPA professional conduct code, Deloitte failed in its duty because it did not conduct itself in the public’s interest. Instead, it gave false audit reports of the company in favor of Rigas family. Second ethical issue The second ethical issue surrounds the company’s executives, specifically Rigas family. Company’s executives should never use their company’s profits for personal gains (Lowenstein, 2004). This is exactlyShow MoreRelatedAdelphia Communications Scandal Revealed Essay782 Words   |  4 PagesADELPHIA COMMUNICATIONS SCANDAL REVEALED ________________________________________ The Adelphia Communications Scandal Created Controversy. 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Trigonometry Mathematics and Theorem Pythagorean Theorem free essay sample

Real Life Applications of Trigonometry A. Importance/Application of Trigonometry in General Fields that use trigonometry or trigonometric functions include astronomy (especially for locating apparent positions of celestial objects, in which spherical trigonometry is essential) and hence navigation (on the oceans, in aircraft, and in space), music theory, acoustics, optics, analysis of financial markets, electronics, probability theory, statistics, biology, medical imaging (CAT scans and ultrasound), pharmacy, chemistry, number theory (and hence cryptology), seismology, meteorology, oceanography, many physical sciences, land surveying and geodesy, architecture, phonetics, economics, electrical engineering, mechanical engineering, civil engineering, computer graphics, cartography, crystallography and game development. B. Pythagorean Theorem Pythagorean Theorem can be use in the field of cartography, mapping out distances between two distinct points or locations. C. Special Right Triangle Theorem Main use for the Special triangle theorems is on the field of architecture or eng ineering as well as geophysics. D. Distance and Midpoint Formula One of the main uses of the distance and midpoint theorem is on the field of optometry. We will write a custom essay sample on Trigonometry: Mathematics and Theorem Pythagorean Theorem or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page It involves the calculation of the lenses curvature related to this medical field. E. Angles and their Measure Geography is one of the main uses for angles and measures. F. Radian Measure One of the primary uses of Radian Measure is on Military Science. Specifically, on Artillery targeting. G. Exponential and Logarithmic Functions For the Exponential Theorem, one of its main uses is on the field of sociology, specifically on population computations, i. e. demographics, exponential decay. This is also use on the field of Finance specifically on the computation of compounded interests. Logarithms can be used mainly on aerospace engineer specifically on the design of airplanes and their components. (i. e. wings, fuselage)